Microgel particles are cross-linked polymer networks that absorb certain liquids causing network expansion. The type of swelling-fluid and extent of volume change depends on the polymer-liquid interaction and the network’s cross-link density. These colloidal gels can be used to stabilize emulsion drops by adsorbing to the interface of two immiscible fluids. However, to enhance the adsorption abilities of these predominantly hydrophilic gel particles, some degree of hydrophobicity is needed. An amphiphilic Janus microgel with spatially distinct lipophilic and hydrophilic sides is desired. Here, we report the fabrication of polyethylene glycol diacrylate/ polypropylene glycol diacrylate Janus microgels (JM) using microfluidic drop making. The flow streams of the two separate and immiscible monomer solutions are brought into contact and intersected by a third immiscible fluid in a flow-focusing junction to form Janus droplets. The individual droplets are crosslinked via UV irradiation to form monodispersed microgel particles with opposing hydrophilic and hydrophobic 3D-networked polymer matrices. By combining two chemically different polymer gel networks, an amphiphilic emulsion stabilizer is formed that adsorbs to the oil/water interface while its faces absorb their respective water or hydrocarbon solvents. Both particle sides swell at the liquid/liquid interface as water in oil emulsions are stabilized and destabilized via thermal responsive hydrogel. Stimuli responsive droplets are demonstrated by adding a short chain oligo ethylene glycol acrylate molecule to the hydrogel formulation on the Janus microgel particle. Droplets stabilized by these particles experience a sudden increase in droplet diameter around 60˚C. This work with absorbent particles may prove useful for applications in bio catalysis, fuel production, and oil transportation.
Many proteins are present at low concentrations in biological samples, and therefore, techniques for ultrasensitive protein detection are necessary. To overcome challenges with sensitivity, the digital enzyme-linked immunosorbent assay (ELISA) was developed, which is 1000× more sensitive than conventional ELISA and allows sub-femtomolar protein detection. However, this sensitivity is still not sufficient to measure many proteins in various biological samples, thereby limiting our ability to detect and discover biomarkers. To overcome this limitation, we developed droplet digital ELISA (ddELISA), a simple approach for detecting low protein levels using digital ELISA and droplet microfluidics. ddELISA achieves maximal sensitivity by improving the sampling efficiency and counting more target molecules. ddELISA can detect proteins in the low attomolar range and is up to 25-fold more sensitive than digital ELISA using Single Molecule Arrays (Simoa), the current gold standard tool for ultrasensitive protein detection. Using ddELISA, we measured the LINE1/ORF1 protein, a potential cancer biomarker that has not been previously measured in serum. Additionally, due to the simplicity of our device design, ddELISA is promising for point-of-care applications. Thus, ddELISA will facilitate the discovery of biomarkers that have never been measured before for various clinical applications.
Co‐precipitation is generally refers to the co‐precipitation of two solids and is widely used to prepare active‐loaded nanoparticles. Here, it is demonstrated that liquid and solid can precipitate simultaneously to produce hierarchical core–shell nanocapsules that encapsulate an oil core in a polymer shell. During the co‐precipitation process, the polymer preferentially deposits at the oil/water interface, wetting both the oil and water phases; the behavior is determined by the spreading coefficients and driven by the energy minimization. The technique is applicable to directly encapsulate various oil actives and avoid the use of toxic solvent or surfactant during the preparation process. The obtained core–shell nanocapsules harness the advantage of biocompatibility, precise control over the shell thickness, high loading capacity, high encapsulation efficiency, good dispersity in water, and improved stability against oxidation. The applications of the nanocapsules as delivery vehicles are demonstrated by the excellent performances of natural colorant and anti‐cancer drug‐loaded nanocapsules. The core–shell nanocapsules with a controlled hierarchical structure are, therefore, ideal carriers for practical applications in food, cosmetics, and drug delivery.
Divalent cations behave as effective cross-linkers of intermediate filaments (IFs) such as vimentin IF (VIF). These interactions have been mostly attributed to their multivalency. However, ion-protein interactions often depend on the ion species, and these effects have not been widely studied in IFs. Here, we investigate the effects of two biologically important divalent cations, Zn2+ and Ca2+, on VIF network structure and mechanics in vitro. We find that the network structure is unperturbed at micromolar Zn2+ concentrations, but strong bundle formation is observed at a concentration of 100 μM. Microrheological measurements show that network stiffness increases with cation concentration. However, bundling of filaments softens the network. This trend also holds for VIF networks formed in the presence of Ca2+, but remarkably, a concentration of Ca2+ that is two orders higher is needed to achieve the same effect as with Zn2+, which suggests the importance of salt-protein interactions as described by the Hofmeister effect. Furthermore, we find evidence of competitive binding between the two divalent ion species. Hence, specific interactions between VIFs and divalent cations are likely to be an important mechanism by which cells can control their cytoplasmic mechanics.
Nanomedicines (i.e., Au@CoFeB-Rg3) were developed by conjugating multimode nanohybrids with active ingredients of natural herbs using Au@CoFeB nanoparticles as one model of multimode nanohybrids and the ginsenoside Rg3 as one model of active ingredients of natural herbs. Au@CoFeB nanoparticles were first synthesized using a temperatureprogrammed microfluidics process. Then, the surface of Au@ CoFeB nanoparticles was modified via an amino-silane coupling agent of (3-aminopropyl) trimethoxysilane (APTMS) and then activated by the bifunctional amine-active cross-linker. They were thereafter conjugated to ginsenosides preactivated by APTMS by cross-linking the surface-activated nanohybrids, forming Au@ CoFeB-Rg3 nanomedicines. Their multimode imaging functions were evaluated with the characterization of their magnetic and optical properties and the response to X-ray radiation. They can be optically detected via dark-field microscopy and can be imaged through X-ray computed tomography. They can also be used as magnetic resonance imaging contrast agents with excellent T2-weighted spin−echo imaging effects. Au@CoFeB-Rg3 nanomedicines exhibited distinct cytotoxicity and inhibitory effects on the proliferation of human hepatocellular carcinoma cells (HepG2/C3) and human chronic myeloid leukemia cells (K562) but were less toxic to 3T3 cells than other cells at concentrations more than 200 μg/ mL. However, Au@CoFeB nanoparticles showed markedly lower cytotoxicity and inhibitory effects on the proliferation of these cell lines, particularly at concentrations <100 μg/mL, than Au@CoFeB-Rg3 nanomedicines. Clearly, there is a distinct synergistic effect between nanohybrids and Rg3. Additionally, Au@CoFeB nanohybrids showed almost no toxicity to Jurkat-CT cells at low concentrations (47 μg/mL), indicating that they may be used as multimode nanoprobes at a suitable concentration. These findings provide an efficient alternative for the synthesis of multifunctional antitumor nanomedicines based on multimode nanohybrids and active ingredients of natural resources.
The nanopore size and roughness of nanoporous surface are two critical variables in determining stem cell fate, but little is known about the contribution from each cue individually. To address this gap, we use two-dimensional nanoporous membranes with controlled nanopore size and roughness to culture bone marrow-derived mesenchymal stem cells (BMSCs), and study their behaviors such as attachment, spreading and differentiation. We find that increasing the roughness of nanoporous surface has no noticeable effect on cell attachment, and only slightly decreases cell spreading areas and inhibits osteogenic differentiation. However, BMSCs cultured on membranes with larger nanopores have significantly fewer attached cells and larger spreading areas. Moreover, these cells cultured on larger nanopores undergo enhanced osteogenic differentiation by expressing more alkaline phosphatase, osteocalcin, osteopontin, and secreting more collagen type I. These results suggest that although both nanopore size and roughness can affect BMSCs, nanopore size plays a more significant role than roughness in controlling BMSC behavior.
Nanoparticle‐shelled bubbles, prepared with glass capillary microfluidics, are functionalized to produce catalytic micromotors that exhibit novel assembly and disassembly behaviors. Stable microbubble rafts are assembled at an air–solvent interface of nonaqueous propylene carbonate (PC) solvent by creating a meniscus using a glass capillary. Upon the addition of hydrogen peroxide fuel, catalytic microbubbles quickly break free from the bubble raft by repelling from each other and self‐propelling at the air–fuel interface (a mixture of PC and aqueous hydrogen peroxide). While most of micromotors generate oxygen bubbles on the outer catalytic shell, some micromotors contain cracks and eject bubbles from the hollow shells containing air. Nanoparticle‐shelled bubbles with a high buoyancy force are particularly attractive for studying novel propulsion modes and interactions between catalytic bubble micromotors at air–fuel interfaces.
We numerically investigate the rheological response of a noncoalescing multiple emulsion under a symmetric shear flow. We find that the dynamics significantly depends on the magnitude of the shear rate and on the number of the encapsulated droplets, two key parameters whose control is fundamental to accurately select the resulting nonequilibrium steady states. The double emulsion, for instance, attains a static steady state in which the external droplet stretches under flow and achieves an elliptical shape (closely resembling the one observed in a sheared isolated fluid droplet), while the internal one remains essentially unaffected. Novel nonequilibrium steady states arise in a multiple emulsion. Under low/moderate shear rates, for instance, the encapsulated droplets display a nontrivial planetarylike motion that considerably affects the shape of the external droplet. Some features of this dynamic behavior are partially captured by the Taylor deformation parameter and the stress tensor. Besides a theoretical interest on its own, our results can potentially stimulate further experiments, as most of the predictions could be tested in the lab by monitoring droplets’ shapes and position over time.
We present a multilayer dropmaker geometry that enables the modular fabrication of microfluidic devices containing precisely patterned channel surface wettability. The platform is used for the scalable production of uniform double emulsion drops. , Microfluidic devices enable the production of uniform double emulsions with control over droplet size and shell thickness. However, the limited production rate of microfluidic devices precludes the use of monodisperse double emulsions for industrial-scale applications, which require large quantities of droplets. To increase throughput, devices can be parallelized to contain many dropmakers operating simultaneously in one chip, but this is challenging to do for double emulsion dropmakers. Production of double emulsions requires dropmakers to have both hydrophobic and hydrophilic channels, requiring spatially precise patterning of channel surface wettability. Precise wettability patterning is difficult for devices containing multiple dropmakers, posing a significant challenge for parallelization. In this paper, we present a multilayer dropmaker geometry that greatly simplifies the process of producing microfluidic devices with excellent spatial control over channel wettability. Wettability patterning is achieved through the independent functionalization of channels in each layer prior to device assembly, rendering the dropmaker with a precise step between hydrophobic and hydrophilic channels. This device geometry enables uniform wettability patterning of parallelized dropmakers, providing a scalable approach for the production of double emulsions.
It remains a grand challenge to prepare anisotropic crystal superstructures with sensitive optical properties in polymer science and materials ﬁeld. This study demonstrates that semicrystalline polymers develop into anisotropic hollow spherulitic crystals spontaneously at interfaces of liquid drops. In contrast to conventional spherulites with centrosymmetric optics and grain boundaries, these anisotropic spherulitic crystals have vanished boundary defects, tunable aspect ratios, and noncentrosymmetric, orientationsensitive birefringence. The experimental ﬁnding is elaborated in poly(L-lactic acid) crystals and is further veriﬁed in a broad class of semicrystalline polymers, irrespective of molecular chirality, chemical constitution, or interfacial modiﬁcation. The facile methods and general mechanism revealed in this study shed light on developing new types of optical microdevices and synthesis of anisotropic semicrystalline particles from liquid emulsions.
Here, we demonstrate use of a Mg 2+ -dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. , Here, we demonstrate use of a Mg 2+ -dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.