An algorithm for extracting the network geometry of three-dimensional collagen gels

Citation:

Stein, A. M. ; Vader, D. A. ; Jawerth, L. M. ; Weitz, D. A. ; Sander, L. M. An algorithm for extracting the network geometry of three-dimensional collagen gels. Journal of Microscopy 2008, 232, 463-475. Copy at http://www.tinyurl.com/ld8kkuv

Abstract:

The geometric structure of a biopolymer network impacts its mechanical and biological properties. In this paper, we develop an algorithm for extracting the network architecture of three-dimensional (3d) fluorescently labeled collagen gels, building on the initial work of Wu et al., (2003). Using artificially generated images, the network extraction algorithm is then validated for its ability to reconstruct the correct bulk properties of the network, including fiber length, persistence length, cross-link density, and shear modulus.

Notes:

Times Cited: 27

Last updated on 04/07/2014