Effects of extract of Coreopsis tinctoria Nutt.on leukemia cells K562
The Coreopsis tinctoria Nutt is a natural flower with many beneficial healthy effects. It is widely used as the flower tea and ethnic medicine by ethnic minority in Xinjiang Uygur Autonomous Region in China. Recent studies have shown that the extract from Coreopsis tinctoria flower possess various biological activities including anti-oxidant,anti-inflammatory,anti-hypertensive,anti-diabetes,anti-aging,anti-hyperlipidemia and neuroprotective effect due to the bioactive components such as flavonoids, polysaccharides and volatile oil etc.
Here in our study, in order to well understand the bioactivities of flavonoid in Coreopsis tinctoria and to further explore the applications in the leukemia field, the anti-tumor activity and potential mechanism were investigated. The appearance observation and the powder identification were carried out to assess the quality of the Coreopsis tinctoria. The total flavonoid was extracted from Coreopsis tinctoria by impregnation method and the concentration was determined by UV. High Performance of Liquid Chromatography coupled with the UV detector (HPLC-UV) method was established to quantification of representative flavonoid component——marein. The effects of total flavonoid on leukemia cells K562 was observed by laser confocal microscope. The cell survival rate was detected by flow cytometry. Our preliminary experimental results showed the total flavonoid exhibited cellular anti-tumor activity. The mechanism might be inducing tumor cell apoptosis.
Chinese herbal medicine attracts global attraction for the effective and safe effects. Our study provides the evidence that the extract from the Coreopsis tinctoria can be the potential candidate to treat leukemia. The merit of the combination of Coreopsis tinctoria combined with Imatinib in inhibition leukemia carcinoma in vitro based on microfluidic chip technology will be further studied next step.
Contact: Xinmei Chen xinmeichen@seas.harvard.edu