Citation:
Cao, T. ; Wang, Y. ; Zhao, L. ; Wang, Y. ; Tao, Y. ; Heyman, J. A. ; Weitz, D. A. ; Zhou, Y. ; Zhang, X. A simple mix-and-read bacteria detection system based on DNAzyme and molecular beacon. Chem. Commun. 2019, 55, 7358-7361. Copy at http://www.tinyurl.com/y3rdbc9u
cao2019.pdf | 580 KB |
Date Published:
MayAbstract:
Here, we describe a simple mix-and-read method for the detection of specific bacterial strains that uses a DNAzyme and a molecular beacon to generate a signal. We have greatly improved upon the previously described DNAzyme-based bacteria detection method by eliminating a tedious preparation step while maintaining detection sensitivity.