Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow

Citation:

Polini, A. ; Mele, E. ; Sciancalepore, A. G. ; Girardo, S. ; Biasco, A. ; Camposeo, A. ; Cingolani, R. ; Weitz, D. A. ; Pisignano, D. Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow. Biomicrofluidics 2010, 4. Copy at http://www.tinyurl.com/ka3lvus
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Abstract:

Producing polymeric or hybrid microfluidic devices operating at high temperatures with reduced or no water evaporation is a challenge for many on-chip applications including polymerase chain reaction (PCR). We study sample evaporation in polymeric and hybrid devices, realized by glass microchannels for avoiding water diffusion toward the elastomer used for chip fabrication. The method dramatically reduces water evaporation in PCR devices that are found to exhibit optimal stability and effective operation under oscillating-flow. This approach maintains the flexibility, ease of fabrication, and low cost of disposable chips, and can be extended to other high-temperature microfluidic biochemical reactors. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3481776]

Notes:

Times Cited: 6

Last updated on 04/15/2014