%0 Journal Article %J Lab Chip %D 2014 %T Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter %A Schmid, Lothar %A Weitz, David A %A Franke, Thomas %X

We describe a versatile microfluidic fluorescence-activated cell sorter that uses acoustic actuation to sort cells or drops at ultra-high rates. Our acoustic sorter combines the advantages of traditional fluorescence-activated cell (FACS) and droplet sorting (FADS) and is applicable for a multitude of objects. We sort aqueous droplets{,} at rates as high as several kHz{,} into two or even more outlet channels. We can also sort cells directly from the medium without prior encapsulation into drops; we demonstrate this by sorting fluorescently labeled mouse melanoma cells in a single phase fluid. Our acoustic microfluidic FACS is compatible with standard cell sorting cytometers{,} yet{,} at the same time{,} enables a rich variety of more sophisticated applications.

%B Lab Chip %I The Royal Society of Chemistry %V 14 %P 3710-3718 %G eng %U http://dx.doi.org/10.1039/C4LC00588K %N 19 %R 10.1039/C4LC00588K