%0 Journal Article %J Biomicrofluidics %D 2014 %T A high-throughput cellulase screening system based on droplet microfluidics %A Ostafe, Raluca %A Prodanovic, Radivoje %A Ung, W. Lloyd %A Weitz, David A %A Fischer, Rainer %X

A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.

%B Biomicrofluidics %V 8 %P 041102 %G eng %U http://scitation.aip.org/content/aip/journal/bmf/8/4/10.1063/1.4886771 %R http://dx.doi.org/10.1063/1.4886771