@inbook {Ridge2016389,
	title = {Chapter Fourteen - Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments},
	booktitle = {Intermediate Filament Proteins},
	series = {Methods in Enzymology},
	volume = {568},
	year = {2016},
	pages = {389 - 426},
	publisher = {Academic Press},
	organization = {Academic Press},
	abstract = {
	The type III\&nbsp;intermediate filament\&nbsp;protein\&nbsp;vimentin\&nbsp;was once thought to function mainly as a static structural protein in the\&nbsp;cytoskeleton\&nbsp;of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of\&nbsp;signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption,\&nbsp;photoactivation\&nbsp;and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments,\&nbsp;collagen gel\&nbsp;contraction,\&nbsp;optical-tweezer\&nbsp;active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.




},
	keywords = {Withaferin A},
	issn = {0076-6879},
	doi = {http://dx.doi.org/10.1016/bs.mie.2015.09.036},
	url = {http://www.sciencedirect.com/science/article/pii/S0076687915005546},
	author = {Karen M. Ridge and Dale Shumaker and Am{\'e}lie Robert and Caroline Hookway and Vladimir I. Gelfand and Paul A. Janmey and Jason Lowery and Guo, Ming and Weitz, DavidA. and Edward Kuczmarski and Robert D. Goldman},
	editor = {M. Bishr Omary and Ronald K.H. Liem}
}
