@article {MABI:MABI201500226,
	title = {Microfluidic Production of Alginate Hydrogel Particles for Antibody Encapsulation and Release},
	journal = {Macromolecular Bioscience},
	volume = {15},
	number = {12},
	year = {2015},
	pages = {1641-1646},
	abstract = {
	Owing to their biocompatibility and reduced side effects, natural polymers represent an attractive choice for producing drug delivery systems. Despite few successful examples, however, the production of monodisperse biopolymer-based particles is often hindered by high viscosity of polymer fluids. In this work, we present a microfluidic approach for production of alginate-based particles carrying encapsulated antibodies. We use a triple-flow micro-device to induce hydrogel formation inside droplets before their collection off-chip. The fast mixing and gelation process produced alginate particles with a unique biconcave shape and dimensions of the mammalian cells. We show slow and fast dissolution of particles in different buffers and evaluate antibody release over time.

},
	keywords = {alginate particles, antibody, droplet microfluidics, drug delivery system},
	issn = {1616-5195},
	doi = {10.1002/mabi.201500226},
	url = {https://pubmed.ncbi.nlm.nih.gov/26198619/},
	author = {Mazutis, Linas and Vasiliauskas, Remigijus and Weitz, DavidA.}
}
