@article {Pessi201482, title = {Microfluidics-assisted engineering of polymeric microcapsules with high encapsulation efficiency for protein drug delivery}, journal = {International Journal of Pharmaceutics}, volume = {472}, year = {2014}, pages = {82 - 87}, abstract = {Abstract In this study, microfluidic technology was employed to develop protein formulations. The microcapsules were produced with a biphasic flow to create water{\textendash}oil{\textendash}water (W/O/W) double emulsion droplets with ultrathin shells. Optimized microcapsule formulations containing 1\% (w/w) bovine serum albumin (BSA) in the inner phase were prepared with poly(vinyl alcohol), polycaprolactone and polyethylene glycol. All the particles were found to be intact and with a particle size of 23{\textendash}47\ μm. Furthermore, the particles were monodisperse, non-porous and stable up to 4 weeks. The encapsulation efficiency of \{BSA\} in the microcapsules was 84\%. The microcapsules released 30\% of their content within 168\ h. This study demonstrates that microfluidics is a powerful technique for engineering formulations for therapeutic proteins.}, keywords = {Drug delivery}, issn = {0378-5173}, doi = {http://dx.doi.org/10.1016/j.ijpharm.2014.06.012}, url = {http://www.sciencedirect.com/science/article/pii/S0378517314004323}, author = {Jenni Pessi and H{\'e}lder A. Santos and Inna Miroshnyk and JoukoYliruusi and Weitz, David A and Mirza, Sabiruddin} }