@article {:/content/aip/journal/bmf/8/4/10.1063/1.4886771,
	title = {A high-throughput cellulase screening system based on droplet microfluidics},
	journal = {Biomicrofluidics},
	volume = {8},
	year = {2014},
	pages = {041102 },
	abstract = {
	A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on\&nbsp;microfluidic\&nbsp;sorting. Cellulase activity is detected using a series of coupled\&nbsp;enzymes\&nbsp;leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of\&nbsp;cells\&nbsp;and greater than 90\% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing\&nbsp;cells\&nbsp;from mixtures containing less than 1\% active\&nbsp;cells.

},
	doi = {http://dx.doi.org/10.1063/1.4886771},
	url = {http://scitation.aip.org/content/aip/journal/bmf/8/4/10.1063/1.4886771},
	author = {Ostafe, Raluca and Prodanovic, Radivoje and Ung, W. Lloyd and Weitz, David A and Fischer, Rainer}
}
