@article {155651,
	title = {Nuclear envelope composition determines the ability of neutrophil-type cells to passage through micron-scale constrictions},
	journal = {Journal of Biological Chemistry},
	volume = {288},
	year = {2013},
	pages = {8610-8618},
	abstract = {
	Neutrophils are characterized by their distinct nuclear shape, which is thought to facilitate the transit of these cells through pore spaces less than one-fifth of their diameter. We used human promyelocytic leukemia (HL-60) cells as a model system to investigate the effect of nuclear shape in whole cell deformability. We probed neutrophil-differentiated HL-60 cells lacking expression of lamin B receptor, which fail to develop lobulated nuclei during granulopoiesis and present an in vitro model for Pelger-Huet anomaly; despite the circular morphology of their nuclei, the cells passed through micron-scale constrictions on similar timescales as scrambled controls. We then investigated the unique nuclear envelope composition of neutrophil-differentiated HL-60 cells, which may also impact their deformability; although lamin A is typically down-regulated during granulopoiesis, we genetically modified HL-60 cells to generate a subpopulation of cells with well defined levels of ectopic lamin A. The lamin A-overexpressing neutrophil-type cells showed similar functional characteristics as the mock controls, but they had an impaired ability to pass through micron-scale constrictions. Our results suggest that levels of lamin A have a marked effect on the ability of neutrophils to passage through micron-scale constrictions, whereas the unusual multilobed shape of the neutrophil nucleus is less essential.

},
	url = {https://www.jbc.org/content/288/12/8610},
	author = {Rowat, Amy C. and Jaalouk, Diana E. and Zwerger, Monika and Ung, W. Lloyd and Eydelnant, Irwin A. and Olins, Don E. and Olins, Ada L. and Herrmann, Harald and Weitz, David A and Lammerding, Jan}
}
