@article {1518446,
	title = {Microfluidic Synthesis of Multimode Au@CoFeB-Rg3 Nanomedicines and Their Cytotoxicity and Anti-Tumor Effects},
	journal = {Chemistry Materials},
	volume = {32},
	number = {12},
	year = {2020},
	pages = {5044-5056},
	abstract = {Nanomedicines (i.e., Au@CoFeB-Rg3) were developed by conjugating multimode nanohybrids with active ingredients of natural herbs using Au@CoFeB nanoparticles as one model of multimode nanohybrids and the ginsenoside Rg3 as one model of active ingredients of natural herbs. Au@CoFeB nanoparticles were first synthesized using a temperatureprogrammed microfluidics process. Then, the surface of Au@ CoFeB nanoparticles was modified via an amino-silane coupling agent of (3-aminopropyl) trimethoxysilane (APTMS) and then activated by the bifunctional amine-active cross-linker. They were thereafter conjugated to ginsenosides preactivated by APTMS by cross-linking the surface-activated nanohybrids, forming Au@ CoFeB-Rg3 nanomedicines. Their multimode imaging functions were evaluated with the characterization of their magnetic and optical properties and the response to X-ray radiation. They can be optically detected via dark-field microscopy and can be imaged through X-ray computed tomography. They can also be used as magnetic resonance imaging contrast agents with excellent T2-weighted spin-echo imaging effects. Au@CoFeB-Rg3 nanomedicines exhibited distinct cytotoxicity and inhibitory effects on the proliferation of human hepatocellular carcinoma cells (HepG2/C3) and human chronic myeloid leukemia cells (K562) but were less toxic to 3T3 cells than other cells at concentrations more than 200 μg/ mL. However, Au@CoFeB nanoparticles showed markedly lower cytotoxicity and inhibitory effects on the proliferation of these cell lines, particularly at concentrations \&lt;100 μg/mL, than Au@CoFeB-Rg3 nanomedicines. Clearly, there is a distinct synergistic effect between nanohybrids and Rg3. Additionally, Au@CoFeB nanohybrids showed almost no toxicity to Jurkat-CT cells at low concentrations (47 μg/mL), indicating that they may be used as multimode nanoprobes at a suitable concentration. These findings provide an efficient alternative for the synthesis of multifunctional antitumor nanomedicines based on multimode nanohybrids and active ingredients of natural resources.},
	url = {https://pubs.acs.org/doi/full/10.1021/acs.chemmater.0c00797},
	author = {Zhang, Weixia and Zhao, Xiaoxiong and Yuan, Yuan and Miao, Fenglin and Li, Wengang and Ji, Shaoxia and Huang, Xing and Chen, Xinhua and Jiang, Tianan and Weitz, DavidA. and Song, Yujun}
}
