@article {chowdhury_dendronized_2019,
	title = {Dendronized fluorosurfactant for highly stable water-in-fluorinated oil emulsions with minimal inter-droplet transfer of small molecules},
	journal = {Nature Communications},
	volume = {10},
	number = {1},
	year = {2019},
	pages = {1-10},
	abstract = {
	
		Fluorosurfactant-stabilized microfluidic droplets are widely used as pico- to nanoliter volume reactors in chemistry and biology. However, current surfactants cannot completely prevent inter-droplet transfer of small organic molecules encapsulated or produced inside the droplets. In addition, the microdroplets typically coalesce at temperatures higher than 80 {\textdegree}C. Therefore, the use of droplet-based platforms for ultrahigh-throughput combination drug screening and polymerase chain reaction (PCR)-based rare mutation detection has been limited. Here, we provide insights into designing surfactants that form robust microdroplets with improved stability and resistance to inter-droplet transfer. We used a panel of dendritic oligo-glycerol-based surfactants to demonstrate that a high degree of inter- and intramolecular hydrogen bonding, as well as the dendritic architecture, contribute to high droplet stability in PCR thermal cycling and minimize inter-droplet transfer of the water-soluble fluorescent dye sodium fluorescein salt and the drug doxycycline.
	

},
	issn = {2041-1723},
	doi = {10.1038/s41467-019-12462-5},
	url = {http://www.nature.com/articles/s41467-019-12462-5},
	author = {Chowdhury, Mohammad Suman and Zheng, Wenshan and Kumari, Shalini and Heyman, John and Zhang, Xingcai and Dey, Pradip and Weitz, DavidA. and Haag, Rainer}
}
