DNA sequence analysis with droplet-based microfluidics


Abate, A. R. ; Hung, T. ; Sperling, R. A. ; Mary, P. ; Rotem, A. ; Agresti, J. J. ; Weiner, M. A. ; Weitz, D. A. DNA sequence analysis with droplet-based microfluidics. Lab on a Chip 2013, 13, 4864-4869. Copy at http://www.tinyurl.com/y24sn2pl


Droplet-based microfluidic techniques can form and process micrometer scale droplets at thousands per second. Each droplet can house an individual biochemical reaction, allowing millions of reactions to be performed in minutes with small amounts of total reagent. This versatile approach has been used for engineering enzymes, quantifying concentrations of DNA in solution, and screening protein crystallization conditions. Here, we use it to read the sequences of DNA molecules with a FRET-based assay. Using probes of different sequences, we interrogate a target DNA molecule for polymorphisms. With a larger probe set, additional polymorphisms can be interrogated as well as targets of arbitrary sequence.

Last updated on 03/10/2015