Citation:
Abbaspourrad, A. ; Zhang, H. ; Tao, Y. ; Cui, N. ; Asahara, H. ; Zhou, Y. ; Yue, D. ; Koehler, S. A. ; Ung, W. L. ; Heyman, J. ; et al. Label-free single-cell protein quantification using a drop-based mix-and-read system. Scientific reports 2015, 5 12756. Copy at http://www.tinyurl.com/y5nmc3v9
Abstract:
Quantitative protein analysis of single cells is rarely achieved due to technical difficulties of detecting minute amounts of proteins present in one cell. We develop a mix-and-read assay for drop-based label-free protein analysis of single cells. This high-throughput method quantifies absolute, rather than relative, amounts of proteins and does not involve antibody labeling or mass spectrometry.